Cortactin (CTTN) (N-term) Rabbit Polyclonal Antibody

CAT#: AP54041PU-N

Cortactin (CTTN) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Cortactin (arrow) in HL-60 cell line lysates (35ug/lane) using Cortactin (SRC8) antibody. (N-term).

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  Immunohistochemistry analysis of human breast carcinoma (Formalin-fixed, Paraffin-embedded) using Cortactin (SRC8) antibody. (N-term), followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC; Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  Immunohistochemistry on Paraffin sections: 1/50-1/100.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the N-terminal region (between 16-46aa) of human Cortactin (SRC8)
• Specificity: This antibody recognizes Cortactin (SRC8) at N-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: cortactin
• Database Link:
  Entrez Gene 2017 Human
  UniProt ID Q14247
• Background: SRC8 (CTTN) is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This protein has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this protein contributes to tumor cell invasion and metastasis.
• Synonyms: CTTN, EMS1, Amplaxin, Oncogene EMS1
• Note: Molecular Weight: 61586 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Pathogenic Escherichia coli infection, Tight junction