Syntaxin 1a (STX1A) (N-term) Rabbit Polyclonal Antibody

CAT#: AP54089PU-N

Syntaxin 1a (STX1A) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Syntaxin 1A / STX1A Antibody (N-term) in 293 cell line lysates (35ug/lane). STX1A (arrow) was detected using the purified Pab.

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  Formalin fixed, paraffin embedded skeletal muscle stained with Syntaxin 1A / STX1A Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the STX1A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of 293 cells using Syntaxin 1A / STX1A Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 36~65 amino acids from the N-terminal region of Human STX1A
• Specificity: This antibody recognizes Human Syntaxin 1A / STX1A (N-term).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens syntaxin 1A (STX1A), transcript variant 2
• Database Link:
  Entrez Gene 6804 Human
• Background: This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis.
• Synonyms: Syntaxin-1A, STX1, Neuron-specific antigen HPC-1
• Note: Molecular Weight: 33023 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport