TIMP3 (Center) Rabbit Polyclonal Antibody

CAT#: AP54257PU-N

TIMP3 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of TIMP3 Antibody (Center) in HepG2 cell line lysates (35 ug/lane). TIMP3 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  TIMP3 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1:1;000.
  Western blot: 1:100-1:500.
  Immunohistochemistry on paraffin sections: 1:10-50.
  Flow cytometry: 1:10~50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 119~148 amino acids from the Center region of human TIMP3
• Specificity: This antibody detects TIMP3 (Center).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide affinity purification
• Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens TIMP metallopeptidase inhibitor 3 (TIMP3)
• Database Link:
  Entrez Gene 7078 Human
• Background: TIMP3 is one of inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM).
• Synonyms: Protein MIG-5, TIMP-3
• Note: Molecular Weight: 24145 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein