Topoisomerase II alpha (TOP2A) (C-term) Rabbit Polyclonal Antibody

CAT#: AP54332PU-N

Topoisomerase II alpha (TOP2A) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

TOP2A/Topo? Antibody (C-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the TOP2A/Topo? antibody detected the TOP2A/Topo? protein (arrow).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:1;000.
  Western blot: 1:100~500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1433-1461 amino acids from the C-terminal region of human TOP2A/Topo?.
• Specificity: This antibody detects TOP2A (C-term).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide affinity purification
• Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens topoisomerase (DNA) II alpha 170kDa (TOP2A)
• Database Link:
  Entrez Gene 7153 Human
• Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
• Synonyms: TOP2, Top-2, DNA topoisomerase 2-alpha, DNA topoisomerase II alpha isozyme
• Note: Molecular Weight: 174385 Da

Reference Data

• Protein Families: Druggable Genome