TRPM8 (N-term) Rabbit Polyclonal Antibody

CAT#: AP54365PU-N

TRPM8 (N-term) rabbit polyclonal antibody, Purified

Product Images

TRPM8 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the TRPM8 antibody detected the TRPM8 protein (arrow).

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  TRPM8 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TRPM8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:1;000.
  Western blot: 1:50~100.
  Immunohistochemistry on paraffin sections: 1:50~100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 76-106 amino acids from the N-terminal region of human TRPM8
• Specificity: This antibody detects TRPM8 (N-term).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens transient receptor potential cation channel subfamily M member 8 (TRPM8)
• Database Link:
  Entrez Gene 79054 Human
• Background: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
• Synonyms: TRPP8, Trp-p8, LTRPC6
• Note: Molecular Weight: 127685 Da

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane