DAP12 (TYROBP) (C-term) Rabbit Polyclonal Antibody

CAT#: AP54428PU-N

DAP12 (TYROBP) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of TYROBP Antibody (C-term) in HepG2 cell line lysates (35 ug/lane). TYROBP (arrow) was detected using the purified Pab.

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  TYROBP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TYROBP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  TYROBP Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1:1;000.
  Western blot: 1:100~500.
  Immunohistochemistry on paraffin sections: 1:50~100.
  Flow cytometry: 1:10~50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 84~113 amino acids from the C-terminal region of human TYROBP
• Specificity: This antibody detects TYROBP / DAP12 (C-term).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: TYRO protein tyrosine kinase binding protein
• Database Link:
  Entrez Gene 7305 Human
  UniProt ID O43914
• Background: TYROBP encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL.
• Synonyms: KARAP
• Note: Molecular Weight: 12179 Da

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Natural killer cell mediated cytotoxicity