UPF2 (Center) Rabbit Polyclonal Antibody

CAT#: AP54472PU-N

UPF2 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

UPF2 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35 ug/lane). This demonstrates the UPF2 antibody detected the UPF2 protein (arrow).

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  UPF2 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1:1;000.
  Western blot: 1:100~500.
  Flow cytometry: 1:10~50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 633-660 amino acids from the Central region of human UPF2
• Specificity: This antibody detects UPF2 / RENT2 (Center).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide affinity purification
• Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant 2
• Database Link:
  Entrez Gene 26019 Human
• Background: UPF2 is a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p.
• Synonyms: KIAA1408
• Note: Molecular Weight: 147810 Da