VEGF Receptor 1 (FLT1) Rabbit Polyclonal Antibody

CAT#: AP54509PU-N

VEGF Receptor 1 (FLT1) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of VGFR1 Antibody in MCF-7 cell line lysates (35 ug/lane). VGFR1 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human breast carcinoma reacted with VGFR1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  VGFR1 Antibody flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blot: 1/50-1/100.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: VGFR1 his fusion protein
• Specificity: This antibody detects VEGFR-1 / Flt-1.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: fms related tyrosine kinase 1
• Database Link:
  Entrez Gene 2321 Human
  UniProt ID P17948
• Background: VGFR1 encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes.
• Synonyms: VEGFR1, FLT1, FLT, FRT, VEGF Receptor 1
• Note: Molecular Weight: 150;769 Da

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Endocytosis, Focal adhesion