XBP1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP54580PU-N

XBP1 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

XBP1 Antibody (C-term) western blot analysis in mouse lung tissue lysates (35 ug/lane). This demonstrates the XBP1 antibody detected the XBP1 protein (arrow).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:1;000.
  Western blot: 1:100~500.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 224-252 amino acids from the C-terminal region of human XBP1
• Specificity: This antibody detects XBP1 / TREB5 (C-term).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide affinity purification
• Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens X-box binding protein 1 (XBP1), transcript variant 2
• Database Link:
  Entrez Gene 22433 MouseEntrez Gene 7494 Human
• Background: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
• Synonyms: XBP-1, XBP2
• Note: Molecular Weight: 28695 Da

Reference Data

• Protein Families: Transcription Factors