DOCK5 Rabbit Polyclonal Antibody

CAT#: AP54883SU-N

DOCK5 rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution:

  ELISA.
  Western Blot: 1/200-1/2000.
  Immunohistochemistry: 1/50-1/500.

• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide derived from C-terminal domain of Dock5 protein
• Specificity: This antibody reacts specifically with 23 and 215 kDa Mouse Dock5 proteins.
  Cross reacts with 215 kDa Human protein.
• Formulation: State: Serum
  State: Lyophilized serum
  Preservative: None
• Background: DOCK 5 (dedicator of cytokinesis protein 5) is a 1,870 amino acid protein belonging to the DOCK family of cytokinesis-regulating proteins. This cytoplasmic peripheral membrane protein activates Rac 1 and Rac 2 small GTPases, while presumably acting as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK 5 contains one DHR-1 (CZH-1) domain, one DHR-2 (CZH-2) domain and one SH3 domain. The DHR-2 domain is a putative GEF activity mediator. In mice, spontaneous mutation of the gene encoding DOCK 5 leads to deletion of the DHR-1 domain, which functions to bind phospholipids and assists in protein-protein interactions, resulting in rupture of lens cataract (RLC). Due to siRNA knockdown studies, it is suspected that DOCK 5 may also be an important mediator of CrkII/CrkL regulation of Caco-2 migration and spreading on COL4. There are two isoforms of DOCK 5 that exist as a result of alternative splicing events.