MEK7 (MAP2K7) pSer271 Rabbit Polyclonal Antibody

CAT#: AP55764PU-S

MEK7 (MAP2K7) pSer271 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from 3T3 cells treated with insulin using MAP2K7 (Phospho-Ser271) Antibody.The lane on the right is treated with the antigen-specific peptide.

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  Immunohistochemical analysis of paraffin-embedded humanbrain tissue, using MAP2K7 (Phospho-Ser271) Antibody (left)or the same antibody preincubated with blocking peptide (right).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500~1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50~1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of Serine 271(V-D-S(p)-K-A) derived from Human MAP2K7 (KLH-conjugated)
• Specificity: The antibody detects endogenous levels of MAP2K7 only when phosphorylated at Serine 271.
• Formulation: Phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 60 kDa
• Database Link:
  Entrez Gene 5609 Human
• Background: The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.
• Synonyms: MAPK/ERK kinase 7, JNK-activating kinase 2, c-Jun N-terminal kinase kinase 2, MAPKK 7, NKK2, MEK7, MKK7, MAP kinase kinase 7