Major Basic Protein (PRG2) Mouse Monoclonal Antibody [Clone ID: BMK13]

CAT#: BM116S

Major Basic Protein (PRG2) mouse monoclonal antibody, clone BMK13, Purified

Specifications

Product Data

• Clone Name: BMK13
• Applications: ELISA, IHC, WB
• Recommended Dilution: IHC with fresh frozen tissue (fix in methanol:acetone)
  IHC with formalin fixed paraffin-embedded tissue (treat with 0.1% trypsin before blocking)
  Western blot
  ELISA
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: Specific for eosinophil major basic protein irrespective of the stages of eosinophil activation and can be regarded as an eosinophil marker.
  The antibody will recognize the 23.8 kDa secreted pro-form of MBP (Pregnancy associated MBP) and the 13.8 kDa mature form found in the matrix of the eosinophil large specific granule.
• Formulation: PBS with 0.09% sodium azide as preservative and 0.1% BSA as stabilizer.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A chromatography
• Storage: Store the antibody undiluted at 2 - 8°C for one month or at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5553 Human
• Background: PRG2 is a cytotoxin and helminthotoxin. It also induces noncytolytic histamine release from human basophils and is involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. In pregnancy serum, the proform exists as a disulfide-linked 2:2 heterotetramer with PAPPA, as a disulfide-linked 2:2 heterotetramer with AGT, and as a complex (probably a 2:2:2 heterohexamer) with AGT and C3dg.
• Synonyms: PRG2, Bone marrow proteoglycan, BMPG, EMBP