beta III Tubulin (TUBB3) (N-term) Mouse Monoclonal Antibody [Clone ID: TU-20]

CAT#: BM170F

beta III Tubulin (TUBB3) (N-term) mouse monoclonal antibody, clone TU-20, FITC

Specifications

Product Data

• Clone Name: TU-20
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on Frozen and Paraffin Sections.
  Immunocytotochemistry on Fixed and Permeabilized cells.
  Suggested working dilution is 1/40.
  The conjugate was also successfully used on FFPE Sections using Confocal Microscopy.
  The unconjugated antibody (BM170S/BM170) also works for:
  Flow cytometry.
  ELISA.
  Western Blot.
  Immunocytochemistry.
• Reactivities: All Species
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide
• Specificity: This antibody recognizes the C-terminal peptide sequence ESESQGPK (aa 441-448) of human class III β-tubulin specific for neurones. The antibody is a highly specific marker for neuronal tissue. TU-20 is very useful for the detection of microtubule structures on fixed cells.
  MAb TU-20 is widely cross-reactive among species (recognized epitope conserved within all species).
• Formulation: PBS containing 15 mM Sodium Azide as preservative, approx. pH 7.4
  Label: FITC
  State: Liquid purified Ig fraction
  Label: Conjugated with Fluorescein isothiocyanate under optimum conditions. The reagent is free of unconjugated FITC.
• Concentration: 1.0 mg/ml
• Conjugation: FITC
• Background: The betaIII-tubulin isotype is present dominantly in cells of neuronal origin and it is one of the earliest marker of neuronal differentiation. Class III beta-tubulin, is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The neuron-associated class III beta-tubulin isotype is most abundant in cells of neuronal origin but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
• Synonyms: Tubulin beta-3 chain, Tubulin beta-III, Tubulin beta-4