Complement C5 (C5) (neoepitope) Mouse Monoclonal Antibody [Clone ID: HCC5b.1 (neo)]

CAT#: BM2133

Complement C5 (C5) (neoepitope) mouse monoclonal antibody, clone HCC5b.1 (neo), Purified

Specifications

Product Data

• Clone Name: HCC5b.1 (neo)
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunoblotting (Western blot).
  Cytological material.
  Immunohistochemistry on Frozen Sections (1/10).
  Incubation time: 1h at RT.
• Reactivities: Bovine, Goat, Human, Porcine, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Activated Human Complement Component C5
• Specificity: HCC 5b.1 (neo) allows detection of C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis.
  Does not react with native C5!
  Polypeptide Reacting: Mr 200 000 polypeptide of SDS denaturated complement component C5 and C5b of Mr 185 000; after C5b activation, an Mr 60 000 cleavage product also reacts (weakly).
  Disorders Specifically Detected: Detection of C5b-9 complexes via activated C5, whereas native C5 reacts with antibody HCC 5.1.
• Formulation: PBS
  State: Purified
  State: Lyophilized purified Ig fraction from Ascites.
  Stabilizer:
  0.5% BSA
  
  Preservative:
  0.09 % sodium azide
• Reconstitution Method: Restore with 1 ml distilled water.
• Purification: Affinity Chromatography on Protein A
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens complement C5 (C5), transcript variant 1
• Database Link:
  Entrez Gene 727 Human
• Background: C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190 kDa glycoprotein consisting of a disulphide linked alpha-chain (111 kDa) and beta-chain (75 kDa).
  C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).
• Synonyms: Complement Component 5, CPAMD4
• Note: Antibody HCC 5.1, cat.no. BM5030 reacts with native C5.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus