Complement C5 (C5) (neoepitope) Mouse Monoclonal Antibody [Clone ID: HCC5b.1 (neo)]

CAT#: BM2133

Complement C5 (C5) (neoepitope) mouse monoclonal antibody, clone HCC5b.1 (neo), Purified


USD 320.00

2 Weeks*

Size
    • 50 ug

Product Images

Specifications

Product Data
Clone Name HCC5b.1 (neo)
Applications ELISA, IHC, WB
Recommended Dilution ELISA.
Immunoblotting (Western blot).
Cytological material.
Immunohistochemistry on Frozen Sections
(1/10).
Incubation time: 1h at RT.
Reactivities Bovine, Goat, Human, Porcine, Primate
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Activated Human Complement Component C5
Specificity HCC 5b.1 (neo) allows detection of C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis.
Does not react with native C5!
Polypeptide Reacting: Mr 200 000 polypeptide of SDS denaturated complement component C5 and C5b of Mr 185 000; after C5b activation, an Mr 60 000 cleavage product also reacts (weakly).
Disorders Specifically Detected: Detection of C5b-9 complexes via activated C5, whereas native C5 reacts with antibody HCC 5.1.
Formulation PBS
State: Purified
State: Lyophilized purified Ig fraction from Ascites.
Stabilizer:
0.5% BSA 

Preservative:
0.09 % sodium azide 
Reconstitution Method Restore with 1 ml distilled water.
Purification Affinity Chromatography on Protein A
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens complement C5 (C5), transcript variant 1
Background C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190 kDa glycoprotein consisting of a disulphide linked alpha-chain (111 kDa) and beta-chain (75 kDa).
C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).
Synonyms Complement Component 5, CPAMD4
Note Antibody HCC 5.1, cat.no. BM5030 reacts with native C5.
Reference Data
Protein Families Druggable Genome
Protein Pathways Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.