SPARC Mouse Monoclonal Antibody [Clone ID: N50]

CAT#: BM2202

SPARC mouse monoclonal antibody, clone N50, Azide Free

Specifications

Product Data

• Clone Name: N50
• Applications: ELISA, FC, IF, IHC, R, WB
• Recommended Dilution: RIA.
  ELISA.
  Immunoflourescence.
  Western blot.
  Immunohistochemistry on Frozen Sections. 10μg/ml.
  Immunohistochemistry on Paraffin Sections: ABC staining method (See "Protocols" for more details).
• Reactivities: Bovine, Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Crude bone extract containing native Osteonectin.
• Specificity: Recognizes Human and Bovine bone Osteonectin, Human platelet Osteonectin.
  SPARC produced by various cells grown in culture.
  Cross reacts with Rat Osteonectin.
• Formulation: 50% Glycerol/H2O (vol/vol)
  State: Azide Free
  State: Liquid purified Ig fraction (>95% pure by SDS-PAGE)
  Preservative: None
• Concentration: lot specific
• Purification: Ammonium Sulfate Precipitation, Ion Exchange Chromatography and Gel Filtration.
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 6678 Human
• Background: SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules. Tumor growth is enhanced in mice lacking SPARC due to changes in the ECM that create a more permissive environment for tumor progression.
• Synonyms: ON, Basement-membrane protein 40, BM-40
• Note: Protocol: For Immunohistochemistry we recommend starting with an antibody concentration of 10 µg/ml and then adjusting this concentration to optimize your specific applications. Because this antibody does not react well in the absence of calcium, we recommend that the dilution be made in a buffer containing 2 mM CaCl2.
  Because Calcium and Phosphate are incompatible, we also recommend a buffer of 20 mM Hepes, 0.15 M NaCl, pH 7.4.
  Recommended Incubation times: 1hour at 37°C, overnight (16 hours) at 2-8°C or 4-6 hours at room temperature.
  For detection, we recommend a FITC labelled secondary antibody such as a goat anti-Mouse IgG.