Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: KS19.2(Z105.6)]

CAT#: BM2244

Cytokeratin 19 (KRT19) mouse monoclonal antibody, clone KS19.2(Z105.6), Purified

Specifications

Product Data

• Clone Name: KS19.2(Z105.6)
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot.
  Cytological Material.
  Immunohistochemistry on Frozen Sections:
  Dilution Buffer: Dilute 1/10 with PBS, pH 7.4, for use in Immunohistochemistry.
  Incubation Time: 1 hour at room temperature.
• Reactivities: Amphibian, Bovine, Chicken, Human, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Keratin K19 of Mr 40000 from cultured Human MCF-7 cells
• Specificity: This antibody represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular Ca.!
  Recognizes a Mr 40,000 polypeptide (Cytokeratin 19) of Human glandular epithelia.
  Positive on the following cell lines: MCF-7, RT 112, Detroit 562, RPMI 2650, HT-29, SSC-12.
  Tumors Specifically Detected: All tested adenocarcinoma; cholangio ca. of liver; renal cell ca.; transitional cell ca. of the bladder; ovary ca.; squamous cell ca. of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast ca.; thymoma.
  Negative Species: Mouse, Woodchuck, Chicken and Xenopus.
• Formulation: PBS pH 7.4
  State: Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore with 1.0 ml distilled water.
• Purification: Affinity Chromatography on Protein A
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3880 Human
• Background: Cytokeratin 19 is a type I acidic subfamily of intermediate filament protein that is expressed in stratified and simple type epithelia. Keratin 19 is not expressed in hepatocytes; therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Keratin 19 is often co-expressed with keratin 7.
• Synonyms: Cytokeratin-19, Keratin-19, KRT19, CK19, K19
• Note: Clone Ks 19.2 (Z105.6) is also published as BM 19.21, MAK 19.21