EPCAM Mouse Monoclonal Antibody [Clone ID: HEA125]

CAT#: BM2274XL

EPCAM mouse monoclonal antibody, clone HEA125, Purified


USD 240.00

2 Weeks*

Size
    • 5 ml

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Specifications

Product Data
Clone Name HEA125
Applications IF, IHC, WB
Recommended Dilution Immunoblotting.
Cytological Material.
Immunofluorescence microscopy.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin-Embedded Tissue.
Cellsorting

PretreatmentWith paraffin embedded sections, protease pre-treatment is required prior to antibody application.
Working Dilutions: Ready-to-use for immunohistochemical application.
Incubation Time: 1 h at RT, extended with paraffin.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen HT-29 colon carcinoma cell line.
Specificity Clone HEA125 recognizes Ep-CAM (polypeptide reacting: Mr 40,000 epithelium-specific cell surface glycoprotein).
HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative.
Tested Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far; particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116).
Polypeptide Reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM).
Formulation PBS, pH 7.4
State: Purified
State: Liquid purified IgG fraction
Stabilizer: 0.5% BSA
Preservative: 0.09% Sodium Azide
Purification Affinity Chromatography on Protein A
Storage Store the antibody undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens epithelial cell adhesion molecule (EPCAM)
Background Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.
Synonyms Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18
Reference Data
Protein Families ES Cell Differentiation/IPS, Transmembrane

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