Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: Ks18.04]

CAT#: BM2275P

Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone Ks18.04, Purified

Specifications

Product Data

• Clone Name: Ks18.04
• Applications: IHC, WB
• Recommended Dilution: Western blot.
  Immunohistochemistry on Frozen and Paraffin Sections: 1/20 with PBS.
  Microwave treatment is recommended.
  Incubation time: 1h at 37°C, extended with paraffin sections (overnight at 2-8°C).
• Reactivities: Bovine, Canine, Hamster, Human, Mouse, Porcine, Rat, Sheep, Trout, Zebrafish
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human keratin K18 from HeLa cytoskeletal preparation
• Specificity: Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin.
  Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.
  Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
  Tested Reactivities on Cultured Cell Lines: HeLa and MCF-7.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium azide
• Reconstitution Method: Restore in 1.0 ml distilled water.
• Purification: Affinity Chromatography on Protein A
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3875 Human
• Background: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
• Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18