GFAP Mouse Monoclonal Antibody [Clone ID: GF12.24]

CAT#: BM2287XL

GFAP mouse monoclonal antibody, clone GF12.24, Purified


USD 240.00

2 Weeks*

Size
    • 5 ml

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Specifications

Product Data
Clone Name GF12.24
Applications IF, IHC, WB
Recommended Dilution Immunoblotting.
Immunofluorescence Microscopy.
Cytological Material.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin-Embedded tissue.
Working Dilution: Ready-to-use for immunohistochemical application.
Incubation time: 1 h ar RT, extended with paraffin.
Reactivities Bovine, Human, Mouse, Rat
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Intermediate filament cytoskeleton from cultured human glioma cells.
Specificity GF 12.24 represents an excellent marker for cell typing.
Suitable for prenatal diagnosis of neural tube defects.
Polypeptide reacting: Mr 50 000 glial filament protein GFP.
Tumors specifically reacting: astrocytomas, gangliomas, medulloblastomas, mixed gliomas, certain ependymomas, certain teratomas.
Tested Reactivities on Cultured Cell Lines:
U 333 CG/343MG.
Formulation PBS, pH 7.4
State: Purified
State: Liquid purified IgG fraction
Stabilizer: 0.5% BSA
Preservative: 0.09% Sodium Azide
Purification Affinity Chromatography on Protein A
Storage Store the antibody undiluted at 2-8°C.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens glial fibrillary acidic protein (GFAP), transcript variant 2
Background Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes
Synonyms Glial Fibrillary Acidic Protein
Reference Data
Protein Families ES Cell Differentiation/IPS

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