CD35 (CR1) Mouse Monoclonal Antibody [Clone ID: J3D3]

CAT#: BM2364

CD35 (CR1) mouse monoclonal antibody, clone J3D3, Azide Free

Specifications

Product Data

• Clone Name: J3D3
• Applications: FC, FN, IHC
• Recommended Dilution: Mechanism of degradation of C3b and Analysis of Lymphoid and Myeloid differentiation: Clone J3D3 is a blocking antibody. It inhibits CR1 mediated decay of cells and blocks C3b dependent rosette formation with Lymphocytes.
  Fluorescence Microscopy or Flow Cytometry: 2 µg/5x10⁵ cells/test.
  Immunohistochemistry: 1/25-1/50. Suitable on Frozen sections or cell smears, and on Bouin's fixed, Paraffin-embedded tissues.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified C3b receptor from Human red blood cells.
• Specificity: Reacts with CD35 also knows as C3b Receptor (CR1).
• Formulation: PBS
  State: Azide Free
  State: Lyophilized purified Ig fraction.
  Stabilizer: 1 mg/ml BSA
  Preservative: None
• Reconstitution Method: Restore with 1 ml of distilled water.
• Concentration: lot specific
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: complement component 3b/4b receptor 1 (Knops blood group)
• Database Link:
  Entrez Gene 1378 Human
• Background: This gene encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.
• Synonyms: Complement receptor type 1, CR1, C3b/C4b receptor