CD56 (NCAM1) Mouse Monoclonal Antibody [Clone ID: MEM-188]

CAT#: BM2401PS

CD56 (NCAM1) mouse monoclonal antibody, clone MEM-188, Purified

Product Images

Surface staining of human peripheral blood lymphocytes with anti-CD56 (MEM-188) purified; GAM-APC.

Specifications

Product Data

• Clone Name: MEM-188
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Western Blot.
  Flow Cytometry: 3 µg/ml.
  Immunoprecipitation.
  Immunohistochemistry on Paraffin Sections: 1/50.
  Pretreatment: Heat retrieval (microwave oven) in citrate buffer standard ABC visualization.
  Positive Tissue: Positive membrane-specific reaction in adjacent peripheral nerve Staining of paraprostatic autonomic ganglion.
  Staining technique: Standard ABC technique (DAB+).
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: KG-1 Human acute myelogenous leukaemia cell line
• Specificity: The antibody reacts with a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes.
  It has been suggested that the antibody MEM-188 could react with Rhesus Monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.
• Formulation: PBS, pH 7.4 with 15 mM Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 4684 Human
• Background: CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity.
  CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.
• Synonyms: NCAM-1, N-CAM-1, NCAM