MAP2 Mouse Monoclonal Antibody [Clone ID: AP20]
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Specifications
Product Data | |
Clone Name | AP20 |
Applications | IF, IHC, WB |
Recommended Dilution | Immunohistochemistry on frozen sections: 1/500. Immunofluorescence: 1/500. Western Blotting: 1-2 µg/ml for 2hrs at RT). Immunohistology (Formalin/paraffin): 1-2 µg/ml for 30 min at RT. [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min.] Anti-MAP-2 may be used in studies of neuron structure in both normal and malignant brain tissue. |
Reactivities | Bovine, Chicken, Human, Mouse, Quail, Rat, Xenopus |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Bovine brain microtubule associated protein 2. |
Specificity | Anti-MAP-2 recognizes (Mr 300 kDa)MAP-2 protein from bovine brain. The antibody strongly cross-reacts with MAP-2 from human, rat, mouse, Xenopus, quail and chicken brains. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c). Anti-MAP-2 reacts with dendrites and cell bodies of neurons. |
Formulation | 0.01 M phosphate buffered saline (PBS) pH 7.2, containing, 1.0% BSA and 0.09% sodium azide as a preservative. Label: Biotin State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein G chromatography |
Conjugation | Biotin |
Storage | Store the antibody at 2-8°C. Do not freeze. |
Stability | Shelf life: one year from despatch. |
Gene Name | Homo sapiens microtubule associated protein 2 (MAP2), transcript variant 1 |
Database Link | |
Background | Microtubule Associated Protein 2 exists in two high molecular weight forms (2a and 2b) and a low molecular weight form (2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. |
Synonyms | Microtubule-associated protein 2, MAP2, Neuronal Marker |
Reference Data | |
Protein Families | Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS |
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