MAP2 Mouse Monoclonal Antibody [Clone ID: AP20]

CAT#: BM2472B

MAP2 mouse monoclonal antibody, clone AP20, Biotin

Specifications

Product Data

• Clone Name: AP20
• Applications: IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on frozen sections: 1/500. Immunofluorescence: 1/500. Western Blotting: 1-2 µg/ml for 2hrs at RT). Immunohistology (Formalin/paraffin): 1-2 µg/ml for 30 min at RT. [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min.]
  Anti-MAP-2 may be used in studies of neuron structure in both normal and malignant brain tissue.
• Reactivities: Bovine, Chicken, Human, Mouse, Quail, Rat, Xenopus
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bovine brain microtubule associated protein 2.
• Specificity: Anti-MAP-2 recognizes (Mr 300 kDa)MAP-2 protein from bovine brain. The antibody strongly cross-reacts with MAP-2 from human, rat, mouse, Xenopus, quail and chicken brains. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c). Anti-MAP-2 reacts with dendrites and cell bodies of neurons.
• Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, containing, 1.0% BSA and 0.09% sodium azide as a preservative.
  Label: Biotin
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein G chromatography
• Conjugation: Biotin
• Storage: Store the antibody at 2-8°C. Do not freeze.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens microtubule associated protein 2 (MAP2), transcript variant 1
• Database Link:
  Entrez Gene 17756 MouseEntrez Gene 25595 RatEntrez Gene 4133 Human
• Background: Microtubule Associated Protein 2 exists in two high molecular weight forms (2a and 2b) and a low molecular weight form (2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.
• Synonyms: Microtubule-associated protein 2, MAP2, Neuronal Marker

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS