CD62E (SELE) (+CD62P) Mouse Monoclonal Antibody [Clone ID: 1.2B6]

CAT#: BM311PT

CD62E (SELE) (+CD62P) mouse monoclonal antibody, clone 1.2B6, Purified

Product Images

Staining of thrombin activated human peripheral blood platelets with mouse anti human CD62E/CD62P

Specifications

Product Data

• Clone Name: 1.2B6
• Applications: ELISA, FC, IHC, IP, WB
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 1-10 µg/ml.
  Positive Control: Human Tonsil.
  ELISA: 10 µg/ml.
  Western Blot (Non-reduced only): Use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.
  Flow Cytometry: 10 µg/ml.
  Immunoprecipitation.
• Reactivities: Human, Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human E-Selectin (ELAM-1)
• Specificity: This antibody recognizes the CD62E and CD62P cell surface antigens. The antibody inhibits neutrophil adhesion to E-selectin and to P-selectin. Although previously thought to recognize only Human CD62E, recent data (Goda et al. 2003) shows that this antibody also recognizes Human CD62P binding to a common epitope on these members of the selectin family.
  Clone 1.2B6 reacts with Porcine E-selectin (CD62E) but not with porcine P-selectin (Stocker et al. 200).
• Formulation: Phosphate buffered saline containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens selectin E (SELE)
• Database Link:
  Entrez Gene 6401 Human
• Background: CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.
• Synonyms: SELE, ELAM1, LECAM2

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)