Desmoplakin (DSP) (DP1+DP2) Mouse Monoclonal Antibody [Clone ID: DP2.15]

CAT#: BM371

Desmoplakin (DSP) (DP1+DP2) mouse monoclonal antibody, clone DP2.15, Purified

Specifications

Product Data

• Clone Name: DP2.15
• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting.
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections: Dilute 1/10 with PBS, pH 7.4
  Incubation Time: 1 h at RT.
• Reactivities: Amphibian, Bovine, Canine, Chicken, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bovine Desmoplakin 1 and 2
• Specificity: This antibody reacts to Desmoplakin 1&2.
  It shows distinct punctate membrane staining of different epithelia.
  Tumors Specifically Detected: Primary and metastatic carcinoma and meningioma.
  Polypeptides Reacting: Desmoplakin 1 and 2 (Mr 250 000 and 215 000).
  Reactivity on Tested Cell Lines: Several Human carcinoma cell lines: MCF-7, A-431; Rat MHC1C1 cell line; Bovine cells: MDBK, BMGE.
• Formulation: Final solution contains PBS, pH 7.4
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore in 1 ml distilled water
• Purification: Affinity Chromatography on Protein A
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens desmoplakin (DSP), transcript variant 2
• Database Link:
  Entrez Gene 306871 RatEntrez Gene 1832 Human
• Background: Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.
• Synonyms: DP, DSP, 250/210 kDa paraneoplastic pemphigus antigen

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC)