CD163 Mouse Monoclonal Antibody [Clone ID: ED2]

CAT#: BM4001

CD163 mouse monoclonal antibody, clone ED2, Purified


USD 650.00

5 Days*

Size
    • 250 ug

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Specifications

Product Data
Clone Name ED2
Applications FC, IF, IHC, IP, WB
Recommended Dilution Western blot.
Immunoprecipitation.
Immunofluorescence. 
Flow Cytometry:
Use 10 µl of 1/10-1/100 diluted CD163 antibody to label 106 cells in 100 µl.
Immunohistochemistry on Frozen Sections: 1/50-1/100. 
Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using pronase prior to staining of paraffin sections.
IHC on paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde) - refer to Whiteland et al (Ref.5.)
Histology Positive ControlLiver tissue.
Reactivities Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Rat Spleen cell homogenate.
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0Ag 14 mouse myeloma cell line.
Specificity This antibody recognises the Rat CD163 cell surface glycoprotein, a 175kD molecule also known as ED2. 
It does not react with Human.
Mouse anti Rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990).
Formulation PBS
State: Purified
State: Liquid purified IgG fraction from Tissue Culture Supernatant
Preservative: 0.09% Sodium Azide
Concentration 0.5 mg/ml
Purification Affinity Chromatography on Protein A
Gene Name Homo sapiens CD163 molecule (CD163), transcript variant 1
Background CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells.
CD163 is expressed by approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.
Synonyms M130, Hemoglobin scavenger receptor, Macrophage marker
Reference Data
Protein Families Druggable Genome, Secreted Protein, Transmembrane

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