CD163 Mouse Monoclonal Antibody [Clone ID: ED2]

CAT#: BM4001

CD163 mouse monoclonal antibody, clone ED2, Purified

Product Images

Staining of ED2 antibody on Rat spleen (frozen section).

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  Staining of acetone fixed, cryostat sectioned Rat spleen with Mouse anti Rat CD163

Specifications

Product Data

• Clone Name: ED2
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Western blot.
  Immunoprecipitation.
  Immunofluorescence.
  Flow Cytometry: Use 10 µl of 1/10-1/100 diluted CD163 antibody to label 10⁶ cells in 100 µl.
  Immunohistochemistry on Frozen Sections: 1/50-1/100.
  Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using pronase prior to staining of paraffin sections.
  IHC on paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde) - refer to Whiteland et al (Ref.5.)
  Histology Positive Control: Liver tissue.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat Spleen cell homogenate.
  Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0Ag 14 mouse myeloma cell line.
• Specificity: This antibody recognises the Rat CD163 cell surface glycoprotein, a 175kD molecule also known as ED2.
  It does not react with Human.
  Mouse anti Rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990).
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purification: Affinity Chromatography on Protein A
• Gene Name: Homo sapiens CD163 molecule (CD163), transcript variant 1
• Database Link:
  Entrez Gene 9332 Human
• Background: CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells.
  CD163 is expressed by approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.
• Synonyms: M130, Hemoglobin scavenger receptor, Macrophage marker

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane