CD163 Mouse Monoclonal Antibody [Clone ID: ED2]

CAT#: BM4001F

CD163 mouse monoclonal antibody, clone ED2, FITC


USD 695.00

5 Days*

Size
    • 100 ug

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Specifications

Product Data
Clone Name ED2
Applications FC
Recommended Dilution Flow Cytometry: Use 10 µl of neat-1/10 diluted antiborde to label 106 cells in 100 µl.
Reactivities Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
Specificity This antibody recognizes the CD163 cell surface glycoprotein, a 175 kD molecule also known as ED2.
Mouse anti Rat CD163, clone ED2 recognizes the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985).
A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007).
Mouse anti Rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990).
Formulation PBS, pH 7.2, containing 0.09% Sodium Azide and 1% BSA
Label: FITC
State: Liquid purified IgG fraction
Label: Fluorescein Isothiocyanate Isomer 1
Concentration 0.1 mg/ml
Purification Affinity Chromatography on Protein A
Conjugation FITC
Gene Name Homo sapiens CD163 molecule (CD163), transcript variant 1
Background CD163 is expressed by approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.
Synonyms M130, Hemoglobin scavenger receptor, Macrophage marker
Reference Data
Protein Families Druggable Genome, Secreted Protein, Transmembrane

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