Sftpd (Lectin Domain) Mouse Monoclonal Antibody [Clone ID: VIF11]

CAT#: BM4005

Sftpd (Lectin Domain) mouse monoclonal antibody, clone VIF11, Purified

Product Images

Immunohistochemistry: SP-D antibody (Clone: VIF11) staining of Rat Lung Frozen Section

Specifications

Product Data

• Clone Name: VIF11
• Applications: ELISA, IHC, WB
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 0.5 μg/ml (1/800) for Rat Cryosections and 10-20 μg/ml (1/20-1/40) for Human Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 10-20 μg/ml (1/20-1/40) for Rat Paraffin Sections (microwave pretreatment for antigen retrieval is recommended).
  Suggested Positive Control: Rat lung.
  Has been described to work in ELISA and Western blots.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Rat Surfactant Protein D (SP-D) from bronchoalveolar lavage.
  The epitope is localized in the lectin domain of SP-D.
• Specificity: Clone VI F11 is useful for staining rat and human surfactant protein D .
  Antigen Distribution on Tissues and Cells: Alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative.
  No reaction on Rat skin, colon, kidney and liver.
  Negative Species: Pig.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 5 mg/ml BSA
  Preservative: 0.05% (v/v) Kathon CG
• Reconstitution Method: Restore by adding 0.5 ml distilled water (= 0.4 mg/ml Stock Solution).
• Concentration: 0.4 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Rattus norvegicus surfactant protein D (Sftpd)
• Database Link:
  Entrez Gene 6441 HumanEntrez Gene 25350 Rat
• Background: Surfactant protein D is a Ca²⁺-dependent carbohydrate binding protein with a similar structure as other C-type mammalian lectins such as conglutinin and SP-A. The molecular weight is 620 kD under non reducing conditions and 43 kD under reducing conditions. The function of SP-D is not clearly defined. SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.
• Synonyms: Lung surfactant protein D, SP-D, PSP-D, PSPD, SFTP4, Collectin-7, COLEC7