Sftpd (Lectin Domain) Mouse Monoclonal Antibody [Clone ID: VIF11]

CAT#: BM4005B

Sftpd (Lectin Domain) mouse monoclonal antibody, clone VIF11, Biotin

Product Images

SFTPD antibody staining of Frozen Rat Lung Section (Clone VIF11, Biotin Conjugated)

Specifications

Product Data

• Clone Name: VIF11
• Applications: ELISA, IHC, WB
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 1 µg/ml (1/400) for rat cryosections.
  Immunohistochemistry on Paraffin Sections: Not tested.
  ELISA.
  Western Blots.
  Suggested Positive Control: Rat lung.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified rat surfactant protein D from bronchoalveolar lavage.
• Specificity: Antigen Distribution on Tissue Sections: alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on Rat skin, colon, kidney and liver.
• Formulation: PBS, pH 7.2, 10 mg/ml BSA as a stabilizer and 0.01% Thimerosal as a preservative
  Label: Biotin
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: 0.4 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Rattus norvegicus surfactant protein D (Sftpd)
• Database Link:
  Entrez Gene 6441 HumanEntrez Gene 25350 Rat
• Background: Surfactant protein D (SP-D) is a Ca²⁺-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43kDa in its reduced state, 620kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein.
  SP-D is synthesized and secreted by alveolar epithelial type II cells.
• Synonyms: Lung surfactant protein D, SP-D, PSP-D, PSPD, SFTP4, Collectin-7, COLEC7