Adgre1 Rat Monoclonal Antibody [Clone ID: CI:A3-1]

CAT#: BM4008APC

Adgre1 rat monoclonal antibody, clone CI:A3-1, APC

Product Images

Mouse anti-Macrophage F4/80 antigen antibody Cat.-No BM4008F (5 µg/ml) on Raw 204.4 cells. Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS.

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  Figure 1. Staining of mouse peritoneal macrophages cells with Mouse F4/80 antigen -APC (BM400APC)

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  Mouse anti-Macrophage F4/80 antigen antibody Cat.-No BM4008F on thioglycollate elicited mouse peritoneal macrophages. Purple: BM4008F; Green: Isotype control SM19FS; Percentages reflect % positive after subtraction of negative control, using M1 marker.

Specifications

Product Data

• Clone Name: CI:A3-1
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice.
  Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS-1 mouse myeloma cell line.
• Specificity: This antibody recognises the Mouse F4/80 antigen, a 160 kD glycoprotein expressed by murine macrophages.
  The antibody binds to Macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture.
  The antigen is not expressed by lymphocytes or polymorphonuclear cells.
• Formulation: PBS, pH 7.4
  Label: APC
  State: Lyophilized purified IgG fraction
  Stabilizer: 1% BSA, 5% Sucrose
  Preservative: 0.09% Sodium Azide
  Label: Allophycocyanin
• Reconstitution Method: Restore with 1 ml distilled water.
• Purification: Affinity Chromatography on Protein A
• Conjugation: APC
• Gene Name: Mus musculus adhesion G protein-coupled receptor E1 (Adgre1)
• Database Link:
  Entrez Gene 13733 Mouse
• Background: F4/80 antigen is a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with Human EGF module-containing Mucin-like Hormone Receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
• Synonyms: Emr1, Gpf480