Macrophages (Haematopoiesis associated) Rat Monoclonal Antibody [Clone ID: ER-HR3]

CAT#: BM4016T

Macrophages (Haematopoiesis associated) rat monoclonal antibody, clone ER-HR3, Purified

Specifications

Product Data

• Clone Name: ER-HR3
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections.
  Flow Cytometry.
  The typical starting working dilution for the applications is 1/50.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2c
• Clonality: Monoclonal
• Specificity:

  The monoclonal antibody ER-HR3 is reactive to the majority of Mouse monocytes and a subset of mature resident macrophages, especially those located in hemopoietic organs. Whereas all other leukocytes are ER-HR3 negative, up to 70% of circulating monocytes stain positive with this monoclonal antibody. Furthermore, about 30% of bone marrow cells express the antigen. Other ER-HR3 positive macrophages can be found in the splenic red pulp, in the mesenteric lymphoid paracortex, interfollicular areas of Peyer's patches and bone marrow. Few ER-HR3 positive cells are observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the renal medulla. Epidermal Langerhans cells also express the antigen.
  The monoclonal antibody ER-HR3 can be used to identify and localize a very distinct mature tissue macrophage subpopulation found in various mouse organs and its distribution pattern is unique compared to that from other macrophage-specific antibodies. The antibody is especially suitable for ontogenic studies because ER-HR3 positive macrophages are closely related to hemopoietic islands, especially at erythropoietic sites.

• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purification: Protein G Chromatography
• Synonyms: Macrophage marker