Ly75 Rat Monoclonal Antibody [Clone ID: NLDC-145]

CAT#: BM4017B

Ly75 rat monoclonal antibody, clone NLDC-145, Biotin

Product Images

Mouse Spleen Frozen Section stained with Biotin conjugated CD205 / DEC-205 Antibody (Clone NLDC-145).

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Specifications

Product Data

• Clone Name: NLDC-145
• Applications: Assay, FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 3 µg/ml (1/300).
  Recommended Positive Control: Mouse Spleen.
  Has been described to work in FACS.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse lymph node tissue.
• Specificity: Reacts with nonlymphoid dendritic cells: interdigitating cells (IDC), veiled cells and Langerhans cells, thymic epithelial cells.
  Monoclonal antibody NLDC-145 identifies Ia positive interdigitating cells, veiled cells and Langerhans cells of the skin and their in vitro counterparts.
  The antigen is expressed at high levels by dendritic cells and thymic epithelial cells.
  The antigen detected by NLDC-145 is an integral membrane glycoprotein with an apparent mass of 205kDa, also known as DEC-205.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Lyophilized purified IgG fraction
  Stabilizer: 5 mg/ml BSA
  Preservative: 0.05% (v/v) Kathon
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: 0.3 mg/ml (after reconstitution)
• Purification: Affinity Chormatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 205 kDa
• Database Link:
  Entrez Gene 17076 Mouse
• Background: Mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells.
  DEC-205 is apparently a receptor involved in antigen-processing by dendritic cells.
• Synonyms: LY75, CLEC13B, DEC205, gp200-MR6