Ly75 Rat Monoclonal Antibody [Clone ID: NLDC-145]

CAT#: BM4017F

Ly75 rat monoclonal antibody, clone NLDC-145, FITC

Product Images

Table 1. Antigen distribution.

Specifications

Product Data

• Clone Name: NLDC-145
• Applications: Assay, FC, IHC
• Recommended Dilution: Flow Cytometry: Starting dilution is 2 µg to label 10e6 cells.
  Suggested Positive Control: GM-CSF and IL-4 stimulated bone marrow cells.
  Antigen Distribution: See Table 1.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse lymph node tissue.
• Specificity: Reacts with nonlymphoid dendritic cells: interdigitating cells (IDC), veiled cells and Langerhans cells, thymic epithelial cells.
  Monoclonal antibody NLDC-145 identifies Ia positive interdigitating cells, veiled cells and Langerhans cells of the skin and their in vitro counterparts.
  The antigen is expressed at high levels by dendritic cells and thymic epithelial cells.
  The antigen detected by NLDC-145 is an integral membrane glycoprotein with an apparent mass of 205kDa, also known as DEC-205.
• Formulation: PBS, pH 7.2
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 10 mg/ml BSA
  Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purification: Affinity Chromatography
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 17076 Mouse
• Background: Mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells.
  DEC-205 is apparently a receptor involved in antigen-processing by dendritic cells.
• Synonyms: LY75, CLEC13B, DEC205, gp200-MR6
• Note: Molecular weight of antigen: 205 kDa.