MRP8 (S100A8) Mouse Monoclonal Antibody [Clone ID: S13.67]

CAT#: BM4029B

MRP8 (S100A8) mouse monoclonal antibody, clone S13.67, Biotin

Specifications

Product Data

• Clone Name: S13.67
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Frozen Sections: 1 µg/ml (1/200).
  Immunohistochemistry on Paraffin Sections: 5-10 µg/ml (1/20-1/50). No pretreatment for antigen retrieval necessary.
  Suggested Positive Control: Human tonsil.
  Has been described to work in FACS and Dot Blots.
• Reactivities: Human, Porcine, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cultured Human Monocytes.
• Specificity: S 13.67 identifies the Ca2+-binding light subunit of the inflammatory L-1 protein complex, also called S100A8 or Calgranulin A.
  S 13.67 is useful for the identification of the 8kD S100A8 subunit in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. S13.67 detects a very distinct, as yet not characterized epitope of MRP8 (different from the 8-5C2 epitope).
  Antigen Distribution
  Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Myeloid leukaemia stains positively.
  Tissue Sections: In the tissue, MRP-8 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP-8 increasingly during the late stages of inflammation. A low MRP-8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8 / MRP14(TNF-alpha/IL-1 alpha induced inflammation of the skin).
• Formulation: PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
  Label: Biotin
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in 0.5 ml distilled water to make stock solution.
• Concentration: 0.2 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Store the loyphilized antibody at 2-8°C.
  Aliquot and store reconstituted antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: S100 calcium binding protein A8
• Database Link:
  Entrez Gene 6279 Human
• Background: MRP8 is the Ca2+-binding light subunit of Calprotectin. MRP8 forms Ca2+-dependent or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8.
• Synonyms: S100-A8, CAGA, MRP-8, CFAG