EPO Mouse Monoclonal Antibody [Clone ID: AE7A5]

CAT#: BM403

EPO mouse monoclonal antibody, clone AE7A5, Purified

Specifications

Product Data

• Clone Name: AE7A5
• Applications: WB
• Recommended Dilution: Western blot: Use at ~1 µg/ml to detect 10 ng/lane of Human EPO under reducing and non reducing conditions.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: A 26 amino acid synthetic peptide analogue of Erythropoietin / EPO
• Specificity: This antibody is specific for Erythropoetin (EPO) and binds to an Epitope within the first 26 amino acids of the N-terminus of Human urinary and Human recombinant EPO.
• Formulation: Phosphate Buffered Saline (PBS), pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 2056 Human
• Background: EPO is a 30 kDa glycoprotein hormone secreted primarily by the kidney that stimulates bone marrow erythropoiesis in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.
• Synonyms: Epoetin