CD93 Mouse Monoclonal Antibody [Clone ID: X-2]

CAT#: BM4030B

CD93 mouse monoclonal antibody, clone X-2, Biotin

Product Images

Human Placenta Frozen Section stained with Biotin conjugated CD93 / C1QR1 Antibody.

Specifications

Product Data

• Clone Name: X-2
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 0.5 μg/ml (1/400).
  It does not react on routinely processed Paraffin Sections.
  Has been described to works in FACS.
  Recommended Positive Control: Human tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human monocytes.
• Specificity: This Monoclonal antibody clone X-2 recognizes a 110kDa O-sialoglycoprotein on granulocytes, monocytes and endothelial cells. Upon differentiation of cultivated monocytes into macrophages, CDw93 ceases to be expressed on day 5-6, which makes it a very useful marker to differentiate those two cell populations.
  Antigen Distribution
  Isolated Cells: Positive with all granulocytes, with staining increasing transiently upon maturation.
  Antigen expression is increased by TNFα, GM-CSF, (fMLP) treatment. No effect on superoxide production by binding to neutrophils has been noted. PMA stimulation causes down regulation of the antigen. Positive with monocytes following fMLP, TNF, GM-CSF treatment.
  Antigen expression decreases on cultured monocytes. Positive on AML cases with monocytic component. Positive on cell lines HL-60, Namalva, K562, THP-1, U937, negative on HMC-1 (human mast cell line).
  Tissue Sections: The Monoclonal antibody X-2 stains epitheloid cells and Rosai-Dorfman cells and most endothelial cells in the synovial membrane of RA patients.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA
  Preservative: 0.01% Thimerosal
• Reconstitution Method: Restore with 0.5 ml distilled water
• Concentration: 0.2 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 22918 Human
• Background: CD93 a cell-surface glycoprotein and type I membrane protein involve in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.
• Synonyms: MXRA4, CDw93, C1q receptor, MBL receptor, SPA receptor