CD18 (ITGB2) (activation epitope) Mouse Monoclonal Antibody [Clone ID: MEM-148]

CAT#: BM4031APC

CD18 (ITGB2) (activation epitope) mouse monoclonal antibody, clone MEM-148, APC

Product Images

Surface staining of CD18 in human peripheral blood with anti-CD18 (MEM-148) APC.

Specifications

Product Data

• Clone Name: MEM-148
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peripheral blood mononuclear cells
• Specificity: The antibody MEM-148 recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1. CD18 (integrin β2 subunit; β2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
• Formulation: Phosphate buffered saline (PBS) solution containing 15mM sodium azide
  Label: APC
  State: Liquid purified Ig fraction
  Label: Conjugated with cross-linked Allophycocyanin under optimum conditions
• Purification: Size-exclusion chromatography
• Conjugation: APC
• Storage: Store the antibody in the dark at 2-8°C.
  Do Not Freeze!
  Avoid prolonged exposure to light.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 3689 Human
• Background: CD18, integrin β2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (β2) integrins: αLβ2 (CD11a/CD18, LFA-1), αMβ2 (CD11b/CD18, Mac-1, CR3), αXβ2 (CD11c/CD18) and αDβ2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
• Synonyms: Integrin beta-2, MFI7