CD18 (ITGB2) (activation epitope) Mouse Monoclonal Antibody [Clone ID: MEM-148]

CAT#: BM4031S

CD18 (ITGB2) (activation epitope) mouse monoclonal antibody, clone MEM-148, Purified

Product Images

Western Blotting analysis of PMA-activated neutrophils (A) and monocytes (B), using anti-human CD18 (MEM-148). The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).

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  Western Blotting analysis of PMA-activated neutrophils (A) and monocytes (B), using anti-human CD18 (MEM-148). The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).

Specifications

Product Data

• Clone Name: MEM-148
• Applications: FC, FN, IP, WB
• Recommended Dilution: Flow Cytometry: 8 µg/ml; The antibody is excellent marker of activated myeloid cells.
  Immunoprecipitation.
  Western Blot (Non Reducing Conditions).
  Functional Application: The antibody induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peripheral blood mononuclear cells
• Specificity: The antibody recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1. CD18 (integrin b2 subunit; b2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
• Formulation: PBS, pH 7.4, with 15 mM Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by PAGE)
• Concentration: lot specific
• Purification: Protein A Affinity Chromatography
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3689 Human
• Background: CD18, integrin b2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
• Synonyms: Integrin beta-2, MFI7