Integrin alpha 5 (ITGA5) Mouse Monoclonal Antibody [Clone ID: X-6]

CAT#: BM4033B

Integrin alpha 5 (ITGA5) mouse monoclonal antibody, clone X-6, Biotin

Specifications

Product Data

• Clone Name: X-6
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: use 10 µl stock solution to label 10e6 cells.
  Immunohistochemistry on Frozen Sections: 4 µg/ml (1/100).
  Suggested Positive Control: Human tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human Monocytes.
• Specificity: This antibody X-6 is useful for the detection of the Fibronectin Receptor, or CD49e.
  Detects Human granulocytes, monocytes and platelets.
  Cross reactivity: unknown.
  X-6 detects an antigen of 154-180kD by Immunoprecipitation.
  Antigen distribution on Isolated cells and Tissue sections:
  Granulocytes, monocytes; positive on blood monocyte-derived macrophages days 0-28. Dull staining with 95% cord blood CD34+ cells, weak reaction on resting neutrophils. Staining increases with maturation of granulocytes.
  Treatment with fMLP, TNF, GM-CSF and temperature causes positive shift with granulocytes and monocytes. PMA treatment of granulocytes leads to a decrease in the number of positive cells. Positive with cell lines U937, DH39 & MEP, HL-60, NB4, HMC-1.
  X-6 is positive on lining cells in different infiltrations and endothelial cells in the synovial membrane of RA patients. The staining pattern is similar to CD34 in these patients.
• Formulation: Stock solution contains PBS, pH 7.2 with 0.01% Thiomerosal as preservative and 10 mg/ml BSA as stabilizer.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 0.5 ml distilled water
• Concentration: 0.4 mg/ml
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Do not freeze working dilutions
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 3678 Human
• Background: The X-6 antibody is useful for the detection of the fibronectin receptor, or CD49e. CD49e is a component of an adhesion receptor heterodimer (α5β1) to fibronectin and invasin.
  α5β1 recognizes an Arg-Gly-Asp sequence in fibronectin. Binding of fibronectin through α5β1 is important for cell survival and induces apoptosis, particularly in hemopoietic cells.
• Synonyms: Integrin alpha-5, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, VLA5, FNRA