CD14 Mouse Monoclonal Antibody [Clone ID: X-8]

CAT#: BM4034

CD14 mouse monoclonal antibody, clone X-8, Purified

Product Images

CD14 antibody staining of Human Placenta Frozen Section.

Specifications

Product Data

• Clone Name: X-8
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 2-4 μg/ml (1/50-1/100).
  Does not react on routinely processed Paraffin Sections.
  Recommended Positive Control: Human placenta.
  Has been described to work in FACS (post fixation is recommended).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human Monocytes.
• Specificity: Monoclonal antibody X-8 prevents the Lipopolysaccharide Binding Protein (LBP) independent activation of cells via soluble CD14 by blocking the binding of CD14.
  BM4034 is specific for Human CD14 on monocytes, some populations of macrophages and granulocytes. The epitope has not been further characterized.
  Antigen Distribution
  Isolated Cells: Monocytes, granulocytes, myeloid leukaemia and myeloid cell lines.
  Tissue Sections: Positive on histiocytes, Langerhans cells, follicular dendritic cells and high endothelial venules.
• Formulation: PBS pH 7.2 containing 10 mg/ml BSA as stabilizer and 0.01% Thimerosal as preservative
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore by adding 0.5 ml distilled water (= Stock solution).
• Concentration: 0.2 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD14 molecule
• Database Link:
  Entrez Gene 929 Human
• Background: CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.
• Synonyms: CD14