CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: 2E1]

CAT#: BM4039

CD32A (FCGR2A) mouse monoclonal antibody, clone 2E1, Purified

Specifications

Product Data

• Clone Name: 2E1
• Applications: ELISA, FC, IHC, IP
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 2 µg/ml (1/200).
  Suggested Positive Control: Human tonsil.
  Has been described to work in ELISA, Immunoprecipitation and FACS (10 µl stock solution to label 10⁶ cells).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: K562 cells.
• Specificity: This Monoclonal Antibody 2E1 recognizes the three isoforms of the Fc-gamma RII designated as A, B and C.
  Monocytes carry all forms of Fc-gamma RII, Neutrophils carry Fc-gamma RIIA and Fc-gamma RIIC. Fc-gamma RIIB is expressed by B lymphocytes and mast cells.
• Formulation: PBS, pH 7.2 containing 0.01% Thiomerosal as preservative and 10 mg/ml BSA as stabilizer
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.5 ml distilled water (stock solution contains 0.4 mg/ml IgG)
• Concentration: 0.4 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2212 Human
• Background: CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.
• Synonyms: Fc-gamma RII-a, Fc-gamma-RIIa, CDw32, FCGR2A, FCG2, FCGR2A1, IGFR2
• Note: Protocol: Protocol with Frozen, ice-cold acetone-fixed sections:
  The whole procedure is performed at room temperature
  1. Wash in PBS
  2. Block endogenous peroxidase
  3. Wash in PBS
  4. Block with 10% normal Goat serum in PBS for 30min. in a humid chamber
  5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  6. Wash in PBS
  7. Incubate with secondary antibody (peroxidase-conjugated Goat anti Mouse IgG+IgM (H+L) minimal-cross reaction to Human) for 1h in a humid chamber
  8. Wash in PBS
  9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  10. Wash in PBS
  11. Counterstain with Mayer's hemalum.