CD33 Mouse Monoclonal Antibody [Clone ID: MY9]

CAT#: BM4040

CD33 mouse monoclonal antibody, clone MY9, Purified

Specifications

Product Data

• Clone Name: MY9
• Applications: FC, IHC, IP
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 4 µg/ml (1/100).
  FACS: 10 µl stock solution to label 10⁶ cells.
  Immunoprecipitation.
  Suggested Positive Control: Human tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: The antigen is CD33
• Specificity: The CD33 antibody is valuable for distinguishing acute myeloid from lymphoblastic
  leukaemias.
  CD33 is expressed on monocytes in peripheral blood. In the bone marrow, CD33 antigen is found on granulocyte and macrophage precursors, and is absent from pluripotent stem cells.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 5 mg/ml BSA
  Preservative: 0.05% (v/v) Kathon CG
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: 0.4 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD33 molecule
• Database Link:
  Entrez Gene 945 Human
• Background: CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.
  Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67kDa M.W. surface antigen.
  CD33 is a 67kDa single chain transmembrane glycoprotein that belongs to the sialoadhesin family.
• Synonyms: Siglec-3, gp67