CD163 Mouse Monoclonal Antibody [Clone ID: 5C6-FAT]

CAT#: BM4041F

CD163 mouse monoclonal antibody, clone 5C6-FAT, FITC

Specifications

Product Data

• Clone Name: 5C6-FAT
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 50 µl of 1/10-1/20 diluted antibody to label 5x10⁵ cells.
  Suggested Positive Control: Human monocytes.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human CD163.
• Specificity: Monoclonal antibody 5C6 FAT recognizes a membrane Glycoprotein on Monocytes and Macrophages which is expressed in intermediate and late inflammatory stages.
  Antigen Distribution
  Isolated Cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days in culture. Does not react with lymphocytes, granulocytes or platelets.
  Tissue Sections: Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, and cortical macrophages of the thymus are detected.
  Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with monoclonal antibody 25F9 (product Cat.-No BM4022) which is not the case in other tissues.
  The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.
• Formulation: PBS, pH 7.2
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 10 mg/ml BSA
  Preservative: 0.05% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.2 mg/ml
• Purification: Affinity Chromatography
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 9332 Human
• Background: CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.
• Synonyms: M130, Hemoglobin scavenger receptor, Macrophage marker