CD46 Mouse Monoclonal Antibody [Clone ID: 13/42]

CAT#: BM4042

CD46 mouse monoclonal antibody, clone 13/42, Purified


USD 510.00

2 Weeks*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name 13/42
Applications Assay, FC, IHC, IP, WB
Recommended Dilution Flow Cytometry: 5-10 µg/ml of neat antibody to label 1x106 cells.
Immunoprecipitation: 5 µg/200 µl extract from 2x106 cells.
Immunohistochemistry on Frozen Sections: 1-2 µg/ml (1/200-1/400). 
Immunohistochemistry on Paraffin Sections: 8 µg/ml (1/50).
Proteinase K pretreatment for antigen retrieval is recommended.
Suggested Positive Control: Human tonsil.
Has been described to work in FACS, Western Blots and Functional Studies.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human U-251 cells. 
Epitope: Localized in the first two short consensus repeats.
Specificity The monoclonal antibody 13/42 recognizes Human CD46.
The recognized epitope is localized on the first two short consensus repeats.
Cross-reacts with African green monkey.
Antigen Distribution: The antigen is widely expressed on Human cells except erythrocytes. It can also be found as a soluble protein in serum and saliva. Many Human cell lines express CD 46 e.g. U937, HeLa.
Formulation PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a preservative
State: Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore with 0.5 ml distilled water.
Concentration 0.4 mg/ml (after reconstitution)
Purification Affinity Chromatography
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month 
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name CD46 molecule
Background CD46 also known as Membrane Cofactor Protein (MCP), acts as a cofactor for factor I, a serine protease which is involved in the degradative cleavage of C3b and C4b. Structurally as well as functionally, it is a member of the regulator of complement activation proteins. CD46 is composed of an amino terminus of four short consensus repeating units (SCR), a Ser/Thr (ST)-rich domain, 13 amino acid residues with unknown function, a transmembrane region, and a cytoplasmic tail. There are many isoforms on human cells and in body fluids which are distinguishable by SDS-PAGE. Their heterogeneity stems from varying amounts of O-linked sugars secondary to alternative splicing of mRNA coding the ST-rich region.
Synonyms TLX, MIC10
Note Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature.
1. Wash in PBS.
2. Block endogenous peroxidase.
3. Wash in PBS.
4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber.
5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber.
6. Wash in PBS.
7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber.
8. Wash in PBS.
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS.
11. Counterstain with Mayer's hemalum.

Protocol with formalin-fixed, paraffin-embedded sections:

The whole procedure is performed at room temperature.
1. Deparaffinize and rehydrate tissue section.
2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS.
6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber.
7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber.
8. Wash in PBS.
9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber.
10. Wash in PBS.
11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
12. Wash in PBS.
13. Counterstain with Mayer's hemalum.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.