CD11a (ITGAL) Mouse Monoclonal Antibody [Clone ID: MEM-25]

CAT#: BM4046R

CD11a (ITGAL) mouse monoclonal antibody, clone MEM-25, PE

Product Images

Surface staining of human peripheral blood cells with anti-human CD11a (MEM-25) PE.

Specifications

Product Data

• Clone Name: MEM-25
• Applications: FC
• Recommended Dilution: Suitable for Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10e6 cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Leukocytes from a pacient suffering from a LGL-type leukaemia.
• Specificity: The antibody MEM-25 reacts with CD11a (α-subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
• Formulation: PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer.
  Label: PE
  State: Liquid purified IgG fraction.
  Label: Conjugated with R-Phycoerythrin under optimum conditions
• Purification: Size-exclusion chromatography
• Conjugation: PE
• Storage: Store the antibody in the dark at 2-8°C.
  Do Not Freeze!
  Avoid prolonged exposure to light.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha L
• Database Link:
  Entrez Gene 3683 Human
• Background: CD11a (LFA-1α) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the αLβ2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
• Synonyms: Integrin alpha-L, LFA1, LFA-1