CD31 (PECAM1) Mouse Monoclonal Antibody [Clone ID: MEM-05]

CAT#: BM4047R

CD31 (PECAM1) mouse monoclonal antibody, clone MEM-05, PE

Product Images

Surface staining of human peripheral blood with anti-human CD31 (MEM-05) PE.

Specifications

Product Data

• Clone Name: MEM-05
• Applications: FC
• Recommended Dilution: Suitable for Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Leukocytes of patient suffering from LGL-type leukaemia
• Specificity: The antibody MEM-05 reacts with CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.
• Formulation: PBS containing 15 mM sodium azide as preservative and 0.2 % (w/v) high-grade BSA (Protease free) as stabilizer.
  Label: PE
  State: Liquid purified Ig fraction
  Label: Conjugated with R-Phycoerythrin under optimum conditions
• Purification: Size-exclusion chromatography
• Conjugation: PE
• Storage: Store the antibody in the dark at 2-8°C.
  Do Not Freeze!
  Avoid prolonged exposure to light.
• Stability: Shelf life: One year from despatch.
• Gene Name: platelet and endothelial cell adhesion molecule 1
• Database Link:
  Entrez Gene 5175 Human
• Background: CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in rebulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
• Synonyms: PECAM-1, EndoCAM, GPIIA'