CD20 (MS4A1) (Cytopl. Dom.) Mouse Monoclonal Antibody [Clone ID: L26]

CAT#: BM4048L

CD20 (MS4A1) (Cytopl. Dom.) mouse monoclonal antibody, clone L26, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human human lymph node stained with CD20 antibody Cat.-No. BM4048L using peroxidase conjugate and DAB chromogen. Note the membrane and cytoplasmic staining of B-cells in mantle and follicle.

Specifications

Product Data

• Clone Name: L26
• Applications: IHC, WB
• Recommended Dilution: Western blot.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/400
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval using high pH target retrieval solution is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Tonsil, lymph node.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human tonsil B cells.
• Specificity: Recognizes
  Cellular Localization: Cell membrane/cytoplsmic.
• Formulation: State: Supernatant
  State: Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD163 molecule (CD163), transcript variant 1
• Database Link:
  Entrez Gene 9332 HumanEntrez Gene 931 Human
• Background: CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.
• Synonyms: MS4A1, Leu-16, Bp35, B-cell marker

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane