Endothelial Cells Rat Monoclonal Antibody [Clone ID: 1F10]

CAT#: BM4057B

Endothelial Cells rat monoclonal antibody, clone 1F10, Biotin

Product Images

Immunohistochemistry on frozen sections on Human placenta with Clone 1F10

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  Distribution of 1F10 antigen in normal and tumor tissues

Specifications

Product Data

• Clone Name: 1F10
• Applications: Assay, IHC
• Recommended Dilution: Immunohistochemistry on Frozen sections: 2 µg/ml (1:200).
  Positive control: Human placenta.
  Does not react on routinely processed paraffin sections.
• Reactivities: Human
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Cultured HUVECs
• Specificity: This antibody recognizes an antigen on human continuous blood endothelial cells which are fully differentiated. The antibody staining is restricted to endothelial cells and does not sta in any other cell in tissues or in peripheral blood. On sinusoidal endothelial cells of the liver or lymphatic organs the antigen is inconsistently expressed or absent.
• Formulation: Phosphate buffered saline pH 7.2
  Label: Biotin
  State: Lyophilized affinity purified Ig fraction
  Stabilizer: 10 mg/ml bovine serum albumin
  Preservative:
  0.01% thimerosal
• Reconstitution Method: Reconstitute by adding 0.5 ml distilled water.
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: The antigen is found in variable numbers on freshly isolated umbilical vein endotheli al cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40 - 70% pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercia lly available endothelial cell media apparently have no influence on the antigen expression.
• Synonyms: Endothelial Cell marker