p53 (TP53) (16-25) Mouse Monoclonal Antibody [Clone ID: BP53-12]

CAT#: BM4079F

p53 (TP53) (16-25) mouse monoclonal antibody, clone BP53-12, FITC

Product Images

Confocal Microscopy of Human HeLa cells using p53 antibody (BP53-12, FITC). The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with the p53-FITC antibody. Photo provided by Dr. Hodny, Inst. of Experimental Medicine, Prague, Czech Republic.

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  Intracellular staining of p53 in RAMOS cells with anti-p53 (BP53-12) FITC.

Specifications

Product Data

• Clone Name: BP53-12
• Applications: FC, IF
• Recommended Dilution: Immunofluorescence: This conjugate is routinely tested by Flow Cytometry analysis using permeabilized transient wild-type p53-tranfectants.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Bacterially expressed full-length wild-type p53.
• Specificity: This Monoclonal antibody BP53-12 recognizes defined epitope (aa 16-25) on Human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells. It is frequently mutated or inactivated in many types of cancer.
• Formulation: Phosphate buffered saline (PBS), pH~7.4
  Label: FITC
  State: Liquid purified Ig fraction
  Preservative: 15 mM Sodium Azide
  Label: Fluorescein Isothiocyanate.
  The reagent is free of unconjugated FITC.
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8 °C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 7157 Human
• Background: The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
• Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13