p53 (TP53) (16-25) Mouse Monoclonal Antibody [Clone ID: BP53-12]

CAT#: BM4079S

p53 (TP53) (16-25) mouse monoclonal antibody, clone BP53-12, Purified


USD 230.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name BP53-12
Applications ELISA, IF, IHC, IP, WB
Recommended Dilution ELISA.
Western Blotting (Non-reducing conditions):
Recommended Dilution: 1-2 µg/ml, overnight in 4°C. 
Positive Control: RAMOS Human lymphoma cell line
Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
SDS-PAGE: 12% separating gel.
Immunoprecipitation.
Immunocytochemistry.
Immunohistochemistry on Frozen and Paraffin-Embedded Sections.
Reactivities Human, Primate
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Bacterially expressed full-length wild-type p53.
Specificity This Monoclonal antibody BP53-12 recognizes defined epitope (aa 16-25) on Human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells. It is frequently mutated or inactivated in many types of cancer.
Formulation Phosphate buffered saline (PBS), pH~7.4
State: Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Preservative: 15 mM Sodium Azide
Concentration lot specific
Purification Precipitation Methods
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
Synonyms Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.