Sftpd (conformational epitope) Mouse Monoclonal Antibody [Clone ID: IIE11]

CAT#: BM4083B

Sftpd (conformational epitope) mouse monoclonal antibody, clone IIE11, Biotin

Specifications

Product Data

• Clone Name: IIE11
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on frozen sections (1 µg/ml; 1:400).
  Immunohistochemistry on paraffin sections (1:100 freshly prepared for staining rat tissues. Fixation: Acetone 5 min 4 °C, paraformaldehyde / paraffin. Tissue pretreatment: microwave treatment 2 x 5 min at 750 W).
  Western Blot.
  Suggested positive control: Rat lung.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified rat surfactant protein D from bronchoalveolar lavage
• Specificity: II E11 recognizes a conformational epitope of SP-D. No cross reactivity with rat SP-A.
  Antigen distribution: Tissue sections: alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on rat skin, colon, kidney and liver.
• Formulation: Phosphate buffered saline pH 7.2, (PBS), 5 mg/ml bovine serum albumin (BSA) as a stabilizer, no preservative.
  Label: Biotin
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore by adding 0.5 ml distilled water.
• Concentration: 0.4 mg/ml
• Conjugation: Biotin
• Storage: Store lyophilized at 2 - 8 °C and reconstituted at -20 °C. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Rattus norvegicus surfactant protein D (Sftpd)
• Database Link:
  Entrez Gene 6441 HumanEntrez Gene 25350 Rat
• Background: Surfactant protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43 kDa in its reduced state, 620 kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.
• Synonyms: Lung surfactant protein D, SP-D, PSP-D, PSPD, SFTP4, Collectin-7, COLEC7